By: Pranoto Direktorat Jenderal PPM & PLP
Keywords: Aedes aegypti; larvae growth; buletin penelitian kesehatanSubject: AEDES; JUVENILE HORMONES
A study to evaluate the efficacy of an insect growth regulator {IAR) methoprene 1.3% (trade name: Allosid G) against Aedes aegypti larvae was conducted in Jakarta from November 1991 through March 1992. The study was conducted by uniformly scattering 1.3% a methoprene in bottom of metal drums filled with 50 liter of regular tap water. Total of 24 drums were utilized for the study with 20 of them given varied dosage rate 0/0.315 gr, 0.625 gr, 1.25 gr, 2.5 gr and 5 gr. Four of the drums without IGR were used for control. One hundred 2 days old larvae of Ae. aegypti were poured into each drum weekly for total of 14 weeks. Also subsequently 25% (total of 12.5 liter) of water from each drum was taken out and replaced with same amount of regular tap water weekly for same period (14 weeks). Observation of larvae growth in the water taken out of the drum was carried by counting the number of dead and alive pupae. Living pupae were transferred into a paper cup covered with mosquito net for further observation number of pupae and adults died and alive. A split plot design was performed using 4 replicates for each application and regression analysis was applied to analysis the data. Efficacy of the IGR in each treatment was calculated through the percentage calculation of efficacy of each replicate devided by four. Weekly efficacy of IGR was calculated as follows: number of died pupae and number of died adults devided by total number of died pupae, number of died adults and number adults alive multiplied by 100%. The trial showed that 100% mortality occured three weeks after treatment, and it decreased from 100% to 91.56% and 99.25% 14 weeks after treatment.
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